Wednesday, October 28, 2020

Medical Research

Collectively, I have about ten years of medical research experience in cancer, genetics, immunology, and virology.

As an undergraduate student, I had four years of experience as laboratory assistant.  I helped developed a model that represent different stages of tumorigenic progression (cancer development). The cells are 1) normal NIH Swiss embryonic cells (mortal); 2) non-tumorigenic NIH 3T3 cells (immortal); 3) tumorigenic ras oncogenic-transformed NIH 3T3 cells; 4) cancer cells (from injection of ras oncogene-transformed NIH 3T3 cells into NIH Swiss mouse; and 5) Metastatic cancer cells (from injection of cancer cells into NIH Swiss mouse).  My undergraduate research involved studying the mitochondrial DNA in tumorigenesis (Federation Proceedings, 48, (1990) 763A. 

After undergraduate, I worked full-time at Children Medical Center in Tulsa studied fragile X syndrome. Our lab used six polymorphic loci, DXS369, DXS297, DXS296, DXS304, IDS and DXS374 to map the fragile X FRAXA chromosome. We report the results of genetic linkage analysis of 32 fragile X [fra(X)] families using 12 polymorphic loci including these new markers. Cytogenetic and molecular data were combined in two-point linkage analysis for the estimation of lod scores and carrier probabilities in potential carriers. Use of these six new marker loci substantially changed the carrier risk estimates for members of 7 of the 32 families from the risk estimates previously calculated on the basis of less closely linked probes available prior to 1989. I was co-author of a publication in this work, appeared in the Amer. J. Medical Genetics 43, 312-319, 1992.

Cancer Immunology
As a graduate student, I had three laboratory rotations involved in the studying viral enhancer in leukemia specificity, cholesterol acyltransferase (ACAT), and gamma interferon MHC class I gene expression. I was a co-author for the study of gamma interferon (Cellular Immunology 150, 90-100, 1993). 

Finally, my thesis examined the epitope variation in CTL-resistant and identifying of minor and major epitopes in murine leukemia viruses. My research these required my independent analyze experimental results, design follow up experiments, modify techniques, and troubleshoot technical problems. I have two publications derived from my research (Viral Immunology 7:51-59, 1994 and J. of General Virology, 76, 635-641, 1995)

After Dartmouth, I went to work for BetaGene, Dallas, Texas for two years involved in deriving several insulin-secreting cell lines, working extensively with molecular genetics, such as cloning, rat-insulin gene knockout, transfecting human insulin gene into rat cell lines, and designing techniques to optimize human insulin excretion in rat cell lines.

Email is the preferred method for initial contact. I am a teacher. I do not answer phone during school hours. Thank you for your understanding.